Expression Of Apoptotic Genes By Polymerase Chain Reaction
FACT CHECK: Did Tata Hospital’s Dr Rajendra Badwe Say Hot Coconut Water Kills Cancer Cells?
Total RNA was isolated from DU-145 cells non treated and treated with different concentration of CKf for 24hrs using RDP Trio Reagent, and cDNA was synthesized using Revert Aid First Stand cDNA synthesis Kit, stored at 20°C. Expression of apoptotic genes, Bax, Bid, Bak, and p53 was performed using the following primers: Bax , Bak p53 Bid . GAPDH was used as an endogenous control. The polymerase chain reaction products were analyzed on a 1.5% agarose gel electrophoresis, and band intensity was detected using Gel Doc-EZ imager . How Can You Keep Your Prostate HealthyThere are effective treatments for benign prostatic hyperplasia. The problem is these treatments often cause side-effects. Dizziness and are two possible side-effects. There are other problematic side-effects too. A man may find that these side-effects are unpleasant. While all men are at risk, older men are more likely to develop an enlarged prostate. Men are looking for ways to reduce their symptoms without the side-effects of drugs used for BPH. For some men, simply keeping their prostate healthy and disease-free is also important. Lets take a closer look at some of the most effective ways in which men can maintain a healthier prostate that functions well, stays small and does not cause any significant problems that could affect their everyday lives. Coconut Oil For The Slim LineThe medium-chain fatty acids in coconut oil, for example, hardly embedded in the fatty tissue and therefore do not make you fat. Instead, they are preferably used for energy. For this purpose, they are converted in the liver into so-called ketones, which in turn can be used by most body cells similar to glucose as fuel. In contrast to glucose, however, the ketones hardly affect the blood sugar level and thus do not lead to an excessive insulin burst as does the glucose. Read Also: When Should You Get Your First Prostate Exam In Vivo Antitumour Effect Of Coconut Water VinegarThe study was conducted according to Yeap et al. , with a slight modification. Dosages of the coconut water vinegar were derived from previous studies, where 0.08 mL/kg body weight was based on 1 Tbsp of vinegar in 250 mL of water, while 2 mL/kg BW has shown in vivo antioxidant and anti-inflammatory effects . Initially, the acclimatised female mice were randomly divided into four groups with eight mice per group. The mice were pretreated with either distilled water or coconut juice vinegar for 6 weeks before being induced with 4T1 cells via subcutaneous injection on week 7, except for the normal control group . The treatment continued for another 28 days before all mice were sacrificed .
The tumours, spleen and blood were collected from the mice. The tumours were weighed and washed in PBS before they were divided into several parts and kept in liquid nitrogen until use. Levels Of Ros: Dcfh da StainingTo determine whether CKf treatment increases the generation of excessive intracellular ROS in DU-145 cells, which may contribute towards cytotoxicity were examined using a fluorescent probe, 2,7-dichlorofluorescein diacetate . 96-well plates seeded with DU-145 cells were treated with different concentrations of CKf for 24h. Treated cells were then washed with phosphate buffered saline and incubated with fresh DCFH-DA in PBS for 30min at 37°C, 5% CO2. After incubation, fresh medium without FBS was added to the wells and fluorescence images were taken using an inverted microscope . You May Like: How Is Radiation For Prostate Cancer Done Determination Of Mitochondrial Superoxide Production: Mitosox StainingDU-145 cells were plated on 96-well plates and incubated overnight to allow for cell attachment. Following treatment with different concentrations of CKf , cells were labelled with MitoSOX Red in complete medium for 20 mins at 37°C. Post incubation, the cell medium was replaced with fresh medium without the dye and cells were incubated for another 20 mins at 37°C. Images were taken by an inverted microscope using either 10/20X objective lens MitoSOX was excited by laser at 514nm . Claim: Coconut Water Is A Virtual Fountain Of Youth And Stops AgingSort Of: Although its true that coconut water is packed with plant hormones called cytokinins it might be a bit far out there to say that it stops aging. The cytokinins in coconut water do support cell division and encourage rapid growth but they wont put aging on hold. It can help to slow the effects of aging, however. Research has suggested that when people consume diets rich in cytokinins, they have a lower risk of developing age related disease and degenerative issues. Coconut water is the richest natural source of cytokinins, so drinking it certainly wont hurt but it wont stop your next birthday from coming. Also Check: What Does Hormone Therapy Do For Prostate Cancer Request An Appointment At Moffitt Cancer CenterPlease call for support from a Moffitt representative. New Patients and Healthcare Professionals can submit an online form by selecting the appropriate button below. Existing patients can call . for a current list of insurances accepted at Moffitt. NEW PATIENTS To request a new patient appointment, please fill out the online form or call 1-888-663-3488. REFERRING PHYSICIANS Providers and medical staff can refer patients by submitting our online referral form. Moffit now offers Virtual Visits for patients. If you are eligible for a virtual appointment, our scheduling team will discuss this option further with you. Moffitt Cancer Center is committed to the health and safety of our patients and their families. For more information on how were protecting our new and existing patients, visit our COVID-19 Info Hub What Should People With Cancer Know About Alkaline Water
#FactCheck: Hot coconut water can cure cancer?
Alkaline water has become a popular drink trend, with many celebrities and fitness gurus touting its hydration and detoxification benefits. You may have also heard that alkaline water can prevent or treat cancer, but no studies have yet proven this to be true. So what should people with cancer know about drinking alkaline water? Here, we talk about what alkaline water is, why the science doesnt support its use for people with cancer, and general guidelines for ways to stay hydrated during your cancer treatment. Also Check: What Causes Prostate Cancer In Young Males What Is The Best Drink For ProstateWhether the prostate is enlarged or still healthy, men want to preserve the well-being of this gland. To do this, the diet itself is essential. Additionally, what you drink impacts your prostate health. In fact, there are a few drinks that may serve as a way of reducing inflammation in the prostate gland. Some of these drinks might be useful for reducing the effects of benign prostatic hyperplasia. Others might help as a preventative strategy against an enlarged prostate. Symptoms Of Prostate EnlargementSeveral symptoms may develop when a man has an enlarged prostate. Men should be on the lookout for these symptoms. Recognizing signs of benign prostatic hyperplasia early on can ensure treatment is initiated before complications develop. The treatment can also help to effectively stop the growth of the prostate. This ensures symptoms do not become more severe. Some of the symptoms a man may experience when they have an enlarged prostate include3:
The effects of an enlarged prostate on the bladder can cause complications. The man is at risk of urinary tract infections. This is due to incomplete emptying of the bladder. In turn, additional symptoms may experience. This could include swollen lymph nodes and fever. Incontinence and acute urinary retention may develop as a complication. Some men may also find that blood appears in their urine. There is also an increase in the risk of bladder stones. This is a complication caused by benign prostatic hyperplasia. There is also a rare risk of experiencing damage to the kidneys and bladder due to an enlarged prostate. Also Check: How Many Stages Does Prostate Cancer Have Ckf Shows The Presence Of Biologically Active ComponentsEarlier studies in our laboratory showed that CKf contained polyphenols and flavonoid class of compounds. Further evaluation using LC-MS showed several peaks. Assessment of each peak and the mass obtained was compared with available literature for confirmation of the compounds. Results showed the presence of coumaric acid, caffeic acid, trihydroxy flavone derivatives, chlorogenic acid, myricetin derivative, tetramer of 5-dihydrobenzoic acid, hydroxy cinnamic acid derivative and titerpene methyl esters in CKf . Fig.1Total ion chromatogram of CKf. a-Coumaric acid, b-Caffeic acid, c-Naringenin, d-Naringenin derivative, e-Chlorogenic acid, f-myricetin derivative, g-Tetramer of 5-dihydrobenzoic acid, h-Hydroxy cinnamic acid derivative, i-Titerpene methyl esters. *Unidentified. No Drinking Hot Coconut Water Does Not Kill Cancer CellsA post claiming hot coconut water kills cancer is being shared across social media platforms attributing the information to Dr. Rajendra Badwe of Tata memorial hospital. Through this article lets fact-check the claim made in the post.
Google search with relevant keywords led us to multiple 2019 news articles which reported the news. According to one such article by Times of India, Tata Memorial Hospital has issued a clarification stating that the claim is unscientific and was never made by its director Dr. Rajendra Badwe. The clarification further added that there is no scientific data to suggest that hot coconut water could cure cancer. Another news article by The Hindu reiterated the same saying that Tata memorial hospital has distanced itself from the viral message, also their clarification rated the information as false and that there is no data to suggest that hot coconut water can provide a cure for any type of cancer. To sum it up, neither Dr. Rajendra Badwe nor Tata memorial hospital had stated that hot coconut water kills cancer cells. Recommended Reading: How To Shrink Your Prostate Naturally How Much Water Do You NeedEach person has unique hydration needs based on variables that include age, gender, weight and the specific treatment plan. A rule of thumb is to aim for at least 64 ounces a day, or eight cups. For a more personalized goal, divide your weight by two to determine how many ounces of fluid to aim for in a day. To translate your daily goal into cups, divide the number of ounces by eight. As long as your tap water is safe to drink, theres no need to buy bottled water. Bottled water is not always better quality. It also costs more, wastes energy and puts millions of empty bottles into the garbage. Tips For Staying HydratedStart when you wake up. Your body needs rehydrating after 6-8 hours of sleep, so keep a glass of water on your nightstand. Keep a reusable water bottle with you. This makes it easier to drink sips throughout the day instead of trying to chug large amounts before bedtime, which can disrupt sleep. Fill a pitcher with your daily consumption. Keep it in your fridge or near your workspace as a reminder. Create a water routine. Develop a schedule of times during the day to refill so you can hold yourself accountable. Use your phones alarm clock to set reminders. Track your progress on an app. There are lots of free options to help you stick to a hydration plan. Use a filtered water pitcher or faucet filter. Filters may improve the taste of tap water by reducing chlorine and other minerals. Read Also: What Type Of Doctor Does Prostate Exams Young Coconut Water: The Functional Drink From NatureImage from :in vitroCan young coconut water be used to replenish the hormone levels in postmenopausal women?Can young coconut water be consumed to supply electrolytes?Can young coconut water prevent cardiovascular disease?Can young coconut water slow the ageing process?Can young coconut water be used in Alzheimer’s disease or dementia?Can young coconut water lower cholesterol in the blood?Does young coconut water improve skin complexion?Does young coconut water improve bone condition?Does young coconut water have any detrimental effect on liver and kidney ? References
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